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Development of a novel subunit vaccine against Mycobacterium avium subspecies paratuberculosis that does not interfere with bovine TB diagnostic

EMIDA-ERA-NET / 2011004 (EMIDA paraTBVaccine)

Project leader
Stig Tollefsen

Summary
Paratuberculosis is a chronic granulomatous enteritis of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP).  Available vaccines against paratuberculosis currently consist of whole bacteria and have shown various efficacies in field studies, but they cannot prevent the animals from becoming bacterial shedders. They also interfere with the diagnosis of paratuberculosis and bovine tuberculosis (TB).

The aim of this project is to develop a novel subunit vaccine for paratuberculosis that does not interfere with diagnostic testing for paratuberculosis and bovine TB. A panel of immunogenic peptides will be identified, some of which will be identified as potential vaccine candidates while others will be used as complementary diagnostic reagents making it possible to combine vaccination with control and eradication programmes for paratuberculosis. Furthermore, this vaccine strategy will not compromise diagnostic tests used in bovine TB disease control programmes. At the end of the project, it is expected that a vaccine candidate that can go on to field evaluation will exist.

Novel immunogenic peptides will be identified by using two completely different complementary approaches, (1) bioinformatics tools (in silico) and (2) generation of T cell lines from peripheral blood and intestinal biopsies from goats naturally infected with paratuberculosis. The generation of T cell lines will be followed by isolation and expansion of single T cell clones. The MAP specific T cell clones will be used to screen for reactivity towards a number of known MAP protein antigens. Formulation, delivery and immunogenicity of vaccines containing multiple peptides as antigens will be determined, and paratuberculosis vaccination and challenge studies will be performed in cattle and goats. The Norwegian Veterinary Institute has the coordinator role in the project and is particularly involved in antigen discovery using T cells and vaccination studies in goats.

Partners

  • Dr. Claus Aagaard, Statens Serum Institut
  • Professor Gregers Jungersen, Technical University of Denmark
  • Dr. Sam Strain, Agri-food and Biosciences Institute

Duration: 31.01.2011 - 28.02.2014

Researchers

Tollefsen Stig

Fungerende seksjonsjef/Forsker

Olsen Ingrid

Forsker

Lybeck Kari

Forsker

Sjurseth Siri Kulberg

Fagansvarlig fjørfehelse/forsker

Heffernan Inger Austrheim

Avdelingsingeniør